BactoReal® Kit Lawsonia intracellularis

 

BactoReal Kit Lawsonia intracellularis

 

BactoReal®  Kit Lawsonia intracellularis

Order no.

Reactions

Pathogen

Internal positive control

DVEB01313

100

FAM channel

Cy5 channel

DVEB01353

50

FAM channel

Cy5 channel

DVEB01311

100

FAM channel

VIC/HEX channel

DVEB01351

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Lawsonia intracellularis
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for L. intracellularis
Water

Background: Lawsonia intracellularis is a Gram-negative and obligate intracellular bacterium and the causative agent of porcine proliferative enteropathy (PPE), which is a transmissible enteric disease of growing pigs characterized by adenomatous proliferation of immature intestinal epithelial cells in the distal small intestines, particularly in the ileum. The infection is widely distributed among pig herds in many pig-producing countries, and affected pigs may exhibit impaired growth and diarrhoea.

Description: BactoReal® Kit Lawsonia intracellularis is based on the amplification and detection of the 16S rRNA gene of L. intracellularis using real-time PCR.

PCR-platforms: BactoReal® Kit Lawsonia intracellularis is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Lawsonia intracellularis


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

preloaderpreloaderpreloaderpreloader