BactoReal® Kit Leptospira spp. (LipL32)

 

BactoReal Kit Leptospira spp LipL32

 

BactoReal®  Kit Leptospira spp. (LipL32)

Order no.

Reactions

Pathogen

Internal positive control

DVEB00613

100

FAM channel

Cy5 channel

DVEB00653

50

FAM channel

Cy5 channel

DVEB00611

100

FAM channel

VIC/HEX channel

DVEB00651

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Leptospira spp.
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for Leptospira
Water

Background: The spirochaetal Leptospira genus consists of pathogenic species (L. interrogans, L. noguchii,   L. weilii, L. kirschneri, L. alexanderi, L. borgpetersenii, L. santarosai, L. kmetyi, Leptospira genomospecies 1), of intermediately pathogenic species (L. inadai, L. fainei, L. broomii, L. licerasiae, L. wolffii) and of non- pathogenic species (L. biflexa, L. meyeri, L. wolbachii, Leptospira genomospecies 3, 4 and 5). Members of Leptospira can also be grouped into serovars. Currently over 200 serovars are recognized in the genus Leptospira and a few serovars are found in more than one species of Leptospira. Non-pathogenic species are saprophytes and can grow outside the host animal, while pathogenic Leptospira species cause leptospirosis. They affect many mammalian species, including humans. Animals may become unapparent carriers, and shedding of leptospires, primarily in the urine, serves as a source of infection for other animals and humans. The clinical signs associated with leptospirosis are variable and depend on the infecting serovar and the susceptibility of the animal. Leptospires are present in the blood during the first 5 to 10 days after onset of the disease. Only pathogenic Leptospira species express an outer membrane lipoprotein encoded by the LipL32 gene.

Description: BactoReal® Kit Leptospira spp. (LipL32) is based on the amplification and detection of the LipL32 gene of pathogenic Leptospira spp. using real-time PCR.

PCR-platforms: BactoReal® Kit Leptospira spp. (LipL32) is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Leptospira spp. (LipL32)


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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