BactoReal® Kit Leptospira spp. Multiplex (16S+LipL32)

 

BactoReal Kit Leptospira spp MultiplexBactoReal FAM_VIC_2 Posko

 

 

BactoReal®  Kit Leptospira spp. Multiplex (16S+LipL32)

Order no.

Reactions

Pathogen

Internal positive control

DVEB00713

100

FAM + VIC/HEX channel

Cy5 channel

DVEB00753

50

FAM + VIC/HEX channel

Cy5 channel

 

Kit contents:
LipL32 detection assay for pathogenic Leptospira spp.
16S rDNA detection assay for pathogenic and intermediately pathogenic Leptospira spp.
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive controls for both Leptospira detection assays
Water

Background: The spirochaetal Leptospira genus consists of pathogenic species (L. interrogans, L. noguchii, L. weilii, L. kirschneri, L. alexanderi, L. borgpetersenii, L. santarosai, L. kmetyi, Leptospira genomospecies 1), of intermediately pathogenic species (L. inadai, L. fainei, L. broomii, L. licerasiae, L. wolffii) and of non- pathogenic species (L. biflexa, L. meyeri, L. wolbachii, Leptospira genomospecies 3, 4 and 5). Members of Leptospira can also be grouped into serovars. Currently over 200 serovars are recognized in the genus Leptospira and a few serovars are found in more than one species of Leptospira. Non-pathogenic species are saprophytes and can grow outside the host animal, while pathogenic Leptospira species cause leptospirosis. They affect many mammalian species, including humans. Animals may become unapparent carriers, and shedding of leptospires, primarily in the urine, serves as a source of infection for other animals and humans. The clinical signs associated with leptospirosis are variable and depend on the infecting serovar and the susceptibility of the animal. Leptospires are present in the blood during the first 5 to 10 days after onset of the disease. Only pathogenic Leptospira species express an outer membrane lipoprotein encoded by the LipL32 gene.

Description: BactoReal® Kit Leptospira spp. Multiplex (16S+LipL32) is based on the detection and differentiation of the LipL32 gene of pathogenic Leptospira species (detection in FAM channel) as well as of the 16S rDNA gene of both intermediately pathogenic and pathogenic Leptospira species (detection in VIC/HEX channel) using multiplex real-time PCR.

PCR-platforms: BactoReal® Kit Leptospira spp. Multiplex (16S+LipL32) is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Leptospira spp. Multiplex (16S rDNA+LipL32)


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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