BactoReal® Kit Mannheimia haemolytica

 

BactoReal Kit Mannheimia haemolyticaBaktoReal Kit FAM_1 Posko

 

BactoReal® Kit Mannheimia haemolytica
Order no. Reactions Pathogen

Internal positive control

DVEB02913

100

FAM channel

Cy5 channel

DVEB02953

50

FAM channel

Cy5 channel

DVEB02911

100

FAM channel

VIC/HEX channel

DVEB02951

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Mannheimia haemolytica
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for Mannheimia haemolytica
Water

Background: Mannheimia haemolytica (formerly Pasteurella haemolytica) is an opportunistic pathogen of cattle, sheep and other ruminants. It causes epizootic pneumonia in cattle known as shipping fever, transit fever or pneumonic pasteurellosis. Mannheimia haemolytica is considered as part of the normal bacterial flora found in the upper respiratory tract of most cattle but is not considered as normal flora of the lungs. It gains access to the lungs when host defenses are compromised by stress or infection with respiratory viruses or mycoplasma.

Description: BactoReal® Kit Mannheimia haemolytica is based on the amplification and detection of the 16S rDNA gene of M. haemolytica using real-time PCR. 

PCR-platforms: BactoReal® Kit Mannheimia haemolytica is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Mannheimia haemolytica


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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