BactoReal® Kit Mycobacterium avium spp. paratuberculosis

 

BactoReal Kit Mycobact avium spp paratubBaktoReal Kit FAM_1 Posko

 

 

BactoReal®  Kit Mycobacterium avium ssp. paratuberculosis

Order no.

Reactions

Pathogen

Internal positive control

DVEB02213

100

FAM channel

Cy5 channel

DVEB02253

50

FAM channel

Cy5 channel

DVEB02211

100

FAM channel

VIC/HEX channel

DVEB02251

50

FAM channel

VIC/HEX channel


Kit contents:
Detection assay for Mycobacterium avium ssp. paratuberculosis
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for M. avium ssp. paratuberculosis
Water

Background: Mycobacterium avium ssp. paratuberculosis (MAP) causes Johne’s disease, a chronic gastroenteritis affecting primarily ruminants and many other species. This disease is one of the most widespread and economically important diseases of ruminants. It has a relatively long incubation period which is characterized by the excretion of MAP in faeces for months and years before first clinical symptoms develop.

Description: BactoReal® Kit Mycobacterium avium ssp. paratuberculosis is based on the amplification and detection of the insertion sequence IS900 of M. avium ssp. paratuberculosis using real-time PCR.

PCR-platforms: BactoReal® Kit Mycobacterium avium ssp. paratuberculosis is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Mycobacterium avium ssp. paratuberculosis


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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