BactoReal® Kit Mycobacterium tuberculosis complex

 

BactoReal Kit Mycobact tuberculosis complBaktoReal Kit FAM_1 Posko

 

 

BactoReal® Kit Mycobacterium tuberculosis complex
Order no. Reactions Pathogen

Internal positive control

DVEB02713

100

FAM channel

Cy5 channel

DVEB02753

50

FAM channel

Cy5 channel

DVEB02711

100

FAM channel

VIC/HEX channel

DVEB02751

50

FAM channel

VIC/HEX channel


Kit contents:
Detection assay for MTBC
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for M. tuberculosis
Water

Background: Tuberculosis in humans is caused by members of the Mycobacterium tuberculosis complex (MTBC). The eight closely related species of the MTBC have a wide range of natural hosts, including human hosts (M. tuberculosis, M. africanum, M. canettii), bovine hosts (M. bovis), caprine hosts (M. caprae), rodent hosts (M. microti), and pinniped hosts (M. pinnipedii). Bovine tuberculosis remains an important disease in many countries of the world, causing significant economic losses.

Description: BactoReal® Kit Mycobacterium tuberculosis complex is based on the amplification and detection of the ITS2 region of Mycobacterium species belonging to the Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. caprae, M. canetti, M. africanum and M. microti) using real-time PCR.

PCR-platforms: BactoReal® Kit Mycobacterium tuberculosis complex is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Mycobacterium tuberculosis complex


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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