BactoReal® Kit Mycoplasma gallisepticum & synoviae

 

BactoReal Kit Mycoplasma gallisepticum_synoviae

 

 

BactoReal® Kit Mycoplasma gallisepticum & synoviae

Order no.

Reactions

Pathogen

Internal positive control

DVEB05713

100

FAM+VIC/HEX channel

Cy5 channel

DVEB05753

50

FAM+VIC/HEX channel

Cy5 channel

 

Kit contents:
– Detection assay for┬áM. gallisepticum and M. synoviae

– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for┬áM. gallisepticum and M. synoviae
– Water

Background: Avian mycoplasmosis is caused by several pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae are the most important avian pathogens. In addition, Mycoplasma meleagridis and Mycoplasma iowae can also cause disease in poultry, but Mycoplasma gallisepticum and Mycoplasma synoviae are considered to be the main species of pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae cause chronic respiratory disease (CRD) and infectious synovitis of chickens and turkeys especially in the presence of stresses or other respiratory pathogens, causing economic losses due to decrease in growth rate, loss of weight and reduced egg production.

Description: BactoReal® Kit Mycoplasma gallisepticum & synoviae is based on the amplification and detection of the mgc2 gene (cytadhesin-like protein encoding gene) of M. gallisepticum and the vlhA gene (haemagglutinin encoding gene) of M. synoviae using multiplex real-time PCR. The mgc2 gene is detected in FAM channel. The vlhA gene is detected in VIC/HEX channel.

PCR-platforms: BactoReal® Kit Mycoplasma gallisepticum & synoviae is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

 


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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