BactoReal® Kit Mycoplasma synoviae

 

BactoReal Kit Mycoplasma synoviaeBaktoReal Kit FAM_1 Posko

 

BactoReal®  Kit Mycoplasma synoviae

Order no.

Reactions

Pathogen

Internal positive control

DVEB00213

100

FAM channel

Cy5 channel

DVEB00253

50

FAM channel

Cy5 channel

DVEB00211

100

FAM channel

VIC/HEX channel

DVEB00251

50

FAM channel

VIC/HEX channel


Kit contents:
Detection assay for Mycoplasma synoviae
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for M. synoviae
Water

Background: Avian mycoplasmosis is caused by several pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae are the most important avian pathogens. In addition, Mycoplasma meleagridis and Mycoplasma iowae can also cause disease in poultry, but Mycoplasma gallisepticum and Mycoplasma synoviae are considered to be the main species of pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae cause chronic respiratory disease (CRD) and infectious synovitis of chickens and turkeys especially in the presence of stresses or other respiratory pathogens, causing economic losses due to decrease in growth rate, loss of weight and reduced egg production.

Description: BactoReal® Kit Mycoplasma synoviae is based on the amplification and detection of the vlhA gene (haemagglutinin encoding gene) gene of M. synoviae using real-time PCR.

PCR-platforms: BactoReal® Kit Mycoplasma synoviae is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Mycoplasma synoviae


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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