BactoReal® Kit Nocardia spp.

 

BactoReal Kit Nocardia sppBaktoReal Kit FAM_1 Posko

 

BactoReal® Kit Nocardia spp.
Order no. Reactions Pathogen

Internal positive control

DVEB03613

100

FAM channel

Cy5 channel

DVEB03653

50

FAM channel

Cy5 channel

DVEB03611

100

FAM channel

VIC/HEX channel

DVEB03651

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Nocardia species
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Nocardia asteroides
– Water

Background: Nocardia is a genus of Gram-positive, facultative intracellular bacteria belonging to the Actinomycetes. Nocardia species are found worldwide in soil, water and plants. This genus has a total of 85 species, of these > 25 have been recognized as causes of human infections, and > 30 are responsible for animal diseases. Nocardia spp. cause opportunistic disease in humans and animals. Nocardiosis is an infectious disease affecting either the lungs, but it may spread to other organ systems, most often the brain and the skin. It may also involve the kidneys, joints, heart, eyes, and bones. Bovine mastitis is the most common clinical manifestation of nocardiosis among domestic ruminants. Nocardial mastitis is observed in dairy herds submitted to inadequate milking management and/or poor hygienic conditions in pre- and post-milking. The most important pathogenic species for humans and animals are N. asteroides complex, N. brasiliensis, N. pseudobrasiliensis, N. otitidiscaviarum and N. transvalensis.

Description: BactoReal® Kit Nocardia spp. is based on the amplification and detection of the 16S rDNA gene of Nocardia spp. using real-time PCR.

PCR-platforms: BactoReal® Kit Nocardia spp. is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Nocardia spp.


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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