BactoReal® Kit Pasteurella multocida

 

BactoReal Kit Pasteurella multocidaBaktoReal Kit FAM_1 Posko

 

BactoReal®  Kit Pasteurella multocida

Order no.

Reactions

Pathogen

Internal positive control

DVEB02813

100

FAM channel

Cy5 channel

DVEB02853

50

FAM channel

Cy5 channel

DVEB02811

100

FAM channel

VIC/HEX channel

DVEB02851

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Pasteurella multocida
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for P. multocida
Water

Background: The Gram-negative bacterium Pasteurella multocida is able to cause disease in a wide range of animal species and in humans. Pasteurella multocida can cause fowl cholera disease in poultry, hemorrhagic septicemia and shipping fever in cattle, atrophic rhinitis in pigs and is the most common cause of respiratory disease, primarily rhinitis, in rabbits. Although Pasteurella multocida is part of the flora in the respiratory tract of pigs, the bacterium can induce hemorrhagic septicemia or, usually as a secondary pathogen invading lungs injured by other bacteria or viruses, pneumonia.  A subset of Pasteurella multocida isolates are critical agents in an upper respiratory disease, progressive atrophic rhinitis (PAR) in pigs.

Description: BactoReal® Kit Pasteurella multocida is based on the amplification and detection of the 16S rRNA gene of P. multocida (subsp. multocida, gallicida, septica and tigris) using real-time PCR. It does not detect other Pasteurella species. For detection of toxigenic P. multocida strains associated with PAR ingenetix offers BactoReal® Kit Pasteurella multocida toxA.

PCR-platforms: BactoReal® Kit Pasteurella multocida is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Pasteurella multocida


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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