BactoReal® Kit Pasteurella multocida toxA

 

BactoReal Kit Pasteurella multocida toxABaktoReal Kit FAM_1 Posko

 

 

BactoReal®  Kit Pasteurella multocida toxA

Order no.

Reactions

Pathogen

Internal positive control

DVEB00913

100

FAM channel

Cy5 channel

DVEB00953

50

FAM channel

Cy5 channel

DVEB00911

100

FAM channel

VIC/HEX channel

DVEB00951

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for toxigenic Pasteurella multocida
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for toxigenic P. multocida
Water

Background: Pasteurella multocida is a Gram-negative bacterium and part of the flora in the respiratory tract of pigs. The bacterium induces hemorrhagic septicemia or, usually as a secondary pathogen invading lungs injured by other bacteria or viruses, pneumonia.  A subset of P. multocida isolates are critical agents in an upper respiratory disease, progressive atrophic rhinitis (PAR). Pasteurella multocida strains associated with PAR usually produce a dermonecrotic toxin, which is encoded by the toxA gene. The toxA protein of these isolates acts as an essential virulence factor inducing turbinate atrophy and poor weight gains in pigs. Non-toxigenic P. multocida strains are not usually associated with PAR. Therefore, confirmation of toxin production is important for the diagnosis and control of the disease.

Description: BactoReal® Kit Pasteurella multocida toxA is based on the amplification and detection of the toxA gene of  P. multocida using real-time PCR. For detection of non-toxigenic P. multocida strains ingenetix offers BactoReal® Kit Pasteurella multocida.

PCR-platforms: BactoReal® Kit Pasteurella multocida toxA is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Pasteurella multocida toxA


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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