BactoReal® Kit Staphylococcus aureus

 

BactoReal Kit Staphylococcus aureusBaktoReal Kit FAM_1 Posko

 

 

BactoReal®  Kit Staphylococcus aureus

Order no.

Reactions

Pathogen

Internal positive control

DVEB01013

100

FAM channel

Cy5 channel

DVEB01053

50

FAM channel

Cy5 channel

DVEB01011

100

FAM channel

VIC/HEX channel

DVEB01051

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Staphylococcus aureus
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for S. aureus
Water

Background: Staphylococcus aureus is a Gram-positive bacterium. In healthy animals, S. aureus colonization of the skin, intestinal tract, or nasopharynx is generally asymptomatic. However, depending on the strain, S. aureus is capable of secreting several exotoxins. Many of these toxins are associated with specific diseases. By producing a toxin, S. aureus is also a common cause of foodborne illness. In cattle, S. aureus causes one of the most common types of chronic mastitis. In humans, S. aureus is a frequent commensal of the skin, but can cause a range of illnesses from minor skin infections to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome and sepsis.

Description: BactoReal® Kit Staphylococcus aureus is based on the amplification and detection of the thermonuclease (nuc) gene of S. aureus using real-time PCR.

PCR-platforms: BactoReal® Kit Staphylococcus aureus is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Staphylococcus aureus


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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