BactoReal® Kit Staphylococcus spp.

 

BactoReal Kit Staphylococcus spp_For research onlyBaktoReal Kit FAM_1 Posko

 

BactoReal® Kit Staphylococcus spp.
Order no. Reactions Pathogen

Internal positive control

DVEB03713

100

FAM channel

Cy5 channel

DVEB03753

50

FAM channel

Cy5 channel

DVEB03711

100

FAM channel

VIC/HEX channel

DVEB03751

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Staphylococcus species
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Staphylococcus
– Water

Background: The genus Staphylococcus includes more than 50 different species. Staphylococci are commensals on skin and upper respiratory tracts of mammals and birds. However, they can also cause various infections of the skin and a wide variety of diseases in humans and animals through either toxin production or penetration. Foreign bodies, such as sutures, indwelling catheters, and implanted joints, are extremely susceptible to Staphylococcus epidermidis colonization, and often serve as the point of entry for infection. Another common path of infection is the respiratory tract, where the bacterium causes pneumonia. Other diseases caused by staphylococci include boils, sinusitis, emesis, diarrhea, endocarditis, scalded skin syndrome, osteomyelitis, urinary tract infection, toxic shock syndrome and food poisoning. Staphylococci, mainly Staphylococcus aureus can also cause bovine mastitis.

Description: BactoReal® Kit Staphylococcus spp. is based on the amplification and detection of the 23S rRNA gene of species of the genus Staphylococcus using real-time PCR.

PCR-platforms: BactoReal® Kit Staphylococcus spp. is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Staphylococcus spp.


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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