BactoReal® Kit Streptococcus agalactiae

 

BactoReal Kit Streptococcus agalactiaeBaktoReal Kit FAM_1 Posko

 

BactoReal®  Kit Streptococcus agalactiae

Order no.

Reactions

Pathogen

Internal positive control

DVEB01513

100

FAM channel

Cy5 channel

DVEB01553

50

FAM channel

Cy5 channel

DVEB01511

100

FAM channel

VIC/HEX channel

DVEB01551

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Streptococcus agalactiae
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for S. agalactiae
Water

Background: Streptococcus agalactiae (also known as Group B streptococcus or GBS) is a beta-hemolytic Gram-positive streptococcus. It is pathogenic primarily for animals, but can also infect humans. Since S. agalactiae strains isolated from animals can be differentiated from those isolated from humans, transmission from animals to humans is rare. In cattle it causes mastitis, an infection of the udder which leads to diminished milk production. Transmission occurs during milking. Streptococcus agalactiae secretes a protein (CAMP factor) which causes haemolysis in the presence of beta-toxin of Staphylococcus aureus in blood agar.

Description: BactoReal® Kit Streptococcus agalactiae is based on the amplification and detection of the cfb gene of S. agalactiae using real-time PCR.

PCR-platforms: BactoReal® Kit Streptococcus agalactiae is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available  as assay only: BactoReal® Streptococcus agalactiae


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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