BactoReal® Kit Streptococcus dysgalactiae

 

BactoReal Kit Streptococcus dysgalactiaeBaktoReal Kit FAM_1 Posko

 

BactoReal®  Kit Streptococcus dysgalactiae

Order no.

Reactions

Pathogen

Internal positive control

DVEB01613

100

FAM + VIC/HEX channel

Cy5 channel

DVEB01653

50

FAM + VIC/HEX channel

Cy5 channel

 

Kit contents:
Multiplex detection assay for Streptococcus dysgalactiae
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for S. dysgalactiae
Water

Background: Streptococcus dysgalactiae is a Gram-positive bacterium belonging to the group C streptococci. It is known to cause clinically acute and subclinical mastitis in cows, ewes and goats, as well as polyarthritis.

Description: BactoReal® Kit Streptococcus dysgalactiae allows the rapid and sensitive detection of S. dysgalactiae (S. dysgalactiae subsp. equisimilis and S. dysgalactiae subsp. dysgalactiae). This test is performed in a multiplex real-time PCR format to enable a differentiation between S. dysgalactiae from other Streptococcus species, especially S. canis. It is based on the amplification and detection of the 23S rRNA gene and 16S rRNA gene. In FAM channel the 23S rRNA gene of S. dysgalactiae and S. canis is detected. In VIC/HEX channel the 16S rRNA gene of several Streptococcus species (S. dysgalactiae, S. agalactiae, S. phocae, S. porcinus, S. didelphis) except that of S. canis is detected. Thus, samples showing a positive signal in FAM as well as in VIC/HEX channel are positive for S. dysgalactiae.

PCR-platforms: BactoReal® Kit Streptococcus dysgalactiae is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: BactoReal® Streptococcus dysgalactiae


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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