BactoReal® Kit Streptococcus spp.

 

BactoReal Kit Streptococcus sppBaktoReal Kit FAM_1 Posko

 

 

BactoReal® Kit Streptococcus spp.

Order no.

Reactions

Pathogen

Internal positive control

DVEB03813

100

FAM channel

Cy5 channel

DVEB03853

50

FAM channel

Cy5 channel

DVEB03811

100

FAM channel

VIC/HEX channel

DVEB03851

50

FAM channel

VIC/HEX channel

 

Kit contents:
– Detection assay for Streptococcus species
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Streptococcus
– Water

Background: Streptococcus is a genus of Gram-positive bacteria belonging to the Firmicutes and the lactic acid bacteria group. There are currently over 50 species known in this genus. Many streptococcal species are nonpathogenic and form part of the commensal animal or human microbiome of the mouth, skin, intestine, and upper respiratory tract. Certain Streptococcus species are responsible for streptococcal pharyngitis, pink eye, meningitis, bacterial pneumonia, endocarditis, erysipelas, necrotizing fasciitis and bovine mastitis.

Description: BactoReal® Kit Streptococcus spp. is based on the amplification and detection of the 23S rRNA gene of species of the genus Streptococcus using real-time PCR.

PCR-platforms: BactoReal® Kit Streptococcus spp. is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: BactoReal® Kit Streptococcus spp. has an analytical sensitivity (defined as the smallest amount of template copies that can be detected) of five copies per PCR. High concentrations of non-Streptococcus species might lead to weak cross reaction. Detection of streptococci by PCR is prone to contamination with streptococcal DNA, which may lead to false-positive results.

Also available as assay only: BactoReal® Streptococcus spp.


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

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