MycoReal Batrachochytrium dendrobatidis

 

MycoReal Batrachochytrium dendrobatidis

 

 

Background: Batrachochytrium dendrobatidis, also known as the amphibian chytrid fungus, causes the disease chytridiomycosis in amphibians. It infects the keratinized skin of amphibians.

MycoReal Batrachochytrium dendrobatidis is a detection assay for DNA of Batrachochytrium dendrobatidis by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: MycoReal Kit Batrachochytrium dendrobatidis

Product MycoReal Batrachochytrium dendrobatidis
Order no. RTGV939
Unit 100 reactions
Technology Real-time PCR
Target gene ITS2 region, detection in FAM channel
Content Assay (pimers + probe) for B. dendrobatidis
Positive Control B. dendrobatidis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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