ParoReal Histomonas meleagridis

 

ParoReal Histomonas meleagridis

 

 

Background: Histomonas meleagridis is a parasitic protozoan causing blackhead disease, infectious enterohepatitis, or histomoniasis. It can infect turkeys, chickens, and occasionally other galliform birds but it is most deadly in turkeys. Chickens are more resistant to the effects of the infection but may act as carriers. Histomonas meleagridis induces extensive and severe necrosis of the tissues of the mucosa and submucosa of cecum and parenchyma of the liver. It is transmitted by the nematode Heterakis gallinarum or directly from the droppings of infected birds. Histomonas meleagridis interacts with other gut organisms, such as bacteria and coccidia, and depends on these for full virulence.

ParoReal Histomonas meleagridis is a detection assay for rapid and sensitive detection of DNA of Histomonas meleagridis by real-time PCR.

Also available as kitversion: ParoReal Kit Histomonas meleagridis

Product ParoReal Histomonas meleagridis
Order no. RTGV267
Unit 100 reactions
Technology Real-time PCR
Target gene 18S rRNA gene, detection in FAM channel
Content Assay (pimers + probe) for Histomonas meleagridis
Positive Control Histomonas meleagridis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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