ParoReal Neospora caninum

 

ParoReal Neospora caninumBakterien Assay Vet FAM

 

Background: Neospora caninum is a coccidian parasite and an important cause of spontaneous abortion in infected livestock. It has a heteroxenous life cycle, with the sexually reproductive stage occurring in the intestine of a definitive host (domestic dog, coyotes, gray wolves and dingos). Oocysts passed in the feces of the definitive host are ingested by intermediate hosts, such as cattle. Formation of cysts results in chronic infection of the intermediate host. Neosporosis is an infectious disease for many different canids and cattle. In canines it leads to neuromuscular degeneration. In dairy and beef cattle it is an important cause of reproductive problems and abortion. Once the cattle is infected, N. caninum is maintained as a life-long infection and is transmissed from cow to the fetus during pregnancy, resulting in abortion or weak or also normal calves. The occurrence of N. caninum infection in beef and dairy cattle has been reported worldwide.

ParoReal Neospora caninum  is a detection assay for rapid and sensitive detection of DNA of Neospora caninum by real-time PCR.

Also available as kitversion: ParoReal Kit Neospora caninum

Product ParoReal Neospora caninum
Order no. RTGV269
Unit 100 reactions
Technology Real-time PCR
Target gene NC5 gene, detection in FAM channel
Content Assay (primers + probe) for N. caninum
Positive Control N. caninum
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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