ParoReal Trichinella spiralis & britovi

 

ParoReal Trichinella_spiralis_britovi

 

Background: Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.

ParoReal Trichinella spiralisĀ & britoviĀ Ā is a detection assay for rapid and sensitive detection of DNA of Trichinella spiralisĀ and T. britoviĀ by real-time PCR. The multiplex-PCR format allows differentiation of both species.

Also available as kitversion: ParoReal Kit Trichinella spiralisĀ & britovi

Product ParoReal Trichinella spiralis & britovi
Order no. RTGV268
Unit 100 reactions
Technology Real-time PCR
Target gene Internal transcribed spacer, detection in FAM & VIC/HEX channel
Content Assay (pimers + probe) for Trichinella spiralis (FAM) &
T. britovi
(VIC/HEX)
Positive Control Trichinella spiralis & britovi
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISMĀ® 7500, LightCyclerĀ® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ā€“ not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
RealĀ 3 (order no. RTGMCR-3) contains primers, a probe and an internal control PCR target included in the assay mix
.
Internal Positive Control Assay 3Ā (order no. RTGMIPC-3) contains primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroRealĀ®, BactoRealĀ® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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