ParoReal Kit Cryptosporidium parvum

 

ParoReal KIT Cryptosporidium parvum

 

 

ParoReal Kit Cryptosporidium parvum
Order no. Reactions Pathogen

Internal positive control

DVEP00613

100

FAM channel

Cy5 channel

DVEP00653

50

FAM channel

Cy5 channel

DVEP00611

100

FAM channel

VIC/HEX channel

DVEP00651

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Cryptosporidium parvum
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for Cryptosporidium parvum
Water

Background: Cryptosporidium parvum is one of several protozoal species that cause cryptosporidiosis, a parasitic disease of the mammalian intestinal tract. It is a zoonotic and obligate intracellular parasite. Primary symptoms of C. parvum infection are acute, watery, and non-bloody diarrhea. C. parvum infection is of particular concern in immunocompromised patients. Extra-intestinal sites include the lung, liver and gall bladder where it causes respiratory cryptosporidosis, hepatitis and cholecystitis. Infection is caused by ingestion of sporulated oocysts transmitted by the faecal-oral route.

Description: ParoReal Kit Cryptosporidium parvum is based on the amplification and detection of the GP60 gene of Cryptosporidium parvum using real-time PCR.

PCR-platforms: ParoReal Kit Cryptosporidium parvum is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: ParoReal Cryptosporidium parvum 


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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