ParoReal Kit Echinococcus multilocularis

 

ParoReal Kit Echinococcus multilocularis

 

 

ParoReal Kit Echinococcus multilocularis   
Order no. Reactions Pathogen

Internal positive control

DVEP00113

100

FAM channel

Cy5 channel

DVEP00153

50

FAM channel

Cy5 channel

DVEP00111

100

FAM channel

VIC/HEX channel

DVEP00151

50

FAM channel

VIC/HEX channel

 

Kit contents:
Detection assay for Echinococcus multilocularis
Detection assay for internal positive control (control of amplification)
DNA reaction mix (contains uracil-N glycosylase, UNG)
Positive control for E. multilocularis
Water

Background: Echinococcus multilocularis is a cyclophyllid tapeworm causing echinococcosis, a disease that affects many canid species including wolves, coyotes, foxes, jackals and domesticated dogs. Humans can also contract this disease. Echinococcus multilocularis requires two types of hosts to mature properly. It is transmitted to intermediate hosts via the ingestion of eggs in infected food. The eggs travel to the lungs, liver, and other internal organs where they develop into multilocular cysts. Finally, Echinococcus multilocularis is transmitted to definitive hosts by means of eating infected, cyst-containing organs. There it becomes attached to the tissues and grows.

Description: ParoReal Kit Echinococcus multilocularis is based on the amplification and detection of the mitochondrial ND1 gene of E. multilocularis using real-time PCR.

PCR-platforms: ParoReal Kit Echinococcus multilocularis is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: ParoReal Echinococcus multilocularis

 ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

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