ParoReal Kit Trichinella spiralis & britovi

 

ParoReal Kit Trichinella_spiralis_britovi

 

ParoReal Kit Trichinella spiralis & britovi

Order no.

Reactions

Pathogen

Internal positive control 

DVEP00413

100

FAM + VIC/HEX channel

Cy5 channel

DVEP00453

50

FAM + VIC/HEX channel

Cy5 channel

 

Kit contents:
– Multiplex detection assay for Trichinella spiralis & T. britovi
– Detection assay for internal positive control (control of amplification)
– DNA reaction mix (contains uracil-N glycosylase, UNG)
– Positive control for Trichinella spiralis & T. britovi
– Water

Background: Trichinellosis is a food-borne parasitic zoonosis caused by nematodes of the genus Trichinella. Twelve genotypes of Trichinella have been identified worldwide four of which are confirmed to exist in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. Two main clades are recognized in the genus: one group (T. britovi, T. murrelli, T. nativa, T. nelsoni, T. spiralis) that encapsulates in host muscle tissue and a second (T. papuae, T. pseudospiralis, T. zimbabwensisi) that does not.

Description: ParoReal Kit Trichinella spiralis & britovi is based on the amplification and detection of the internal transcribed spacer of Trichinella spiralis and T. britovi using real-time PCR.  Trichinella spiralis is detected in FAM channel and Trichinella britovi is detected in VIC/HEX channel.

PCR-platforms: ParoReal Kit Trichinella spiralis & britovi is developed and validated for the ABI PRISM® 7500 instrument (Life Technologies), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Sensitivity and specificity: See product description

Also available as assay only: ParoRealTrichinella spiralis & britovi

 


ViroReal®, BactoReal® and ParoReal Kits run with the same cycling conditions.

RNA and DNA material can be analysed in one PCR run.

 

preloaderpreloaderpreloaderpreloader