BactoReal® Actinobacillus pleuropneumoniae


BactoReal Actinobacillus pleuropneumoniae


Background: Actinobacillus pleuropneumoniae (APP) causes respiratory disease in swine (pig haemorrhagic pleuropneumonia, actinobacillosis). It is a gram-negative coccobacillus respiratory pathogen.  Clinical signs of the acute disease are dyspnea, coughing, anorexia, depression, fever and sometimes vomiting. The disease can progress very rapidly and death can occur within a few hours. Chronic infections are characterized by cough and pleuritis. Many herds are infected with APP without presenting any clinical evidence of the disease. Carrier pigs harbour APP in their nasal cavities and/or tonsils.

BactoReal® Actinobacillus pleuropneumoniae is a detection assay for rapid and sensitive detection of DNA of Actinobacillus pleuropneumoniae by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Actinobacillus pleuropneumoniae

Product BactoReal® Actinobacillus pleuropneumoniae
Order no. RTGV550
Unit 100 reactions
Technology Real-time PCR
Target gene apxIVA gene, detection in FAM channel
Content Assay (pimers + probe) for Actinobacillus pleuropneumoniae
Positive Control Actinobacillus pleuropneumoniae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.