BactoReal® American Foulbrood

 

BactoReal American FoulbroodBakterien Assay Vet Vic

 

Background: American foulbrood (AFB) is a bacterial brood disease of honey bees caused by the Gram-positive and spore-forming bacterium Paenibacillus larvae. The disease is transferred and initiated only by the spore stage of the bacterium. Young bee larvae are infected by spores, then the spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium produces many millions of spores which may remain dormant for many years.

BactoReal® American Foulbrood is a detection assay for rapid and sensitive detection of DNA of Paenibacillus larvae by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit American Foulbrood

Product BactoReal® American Foulbrood
Order no. RTGV924
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in VIC/HEX channel
Content Assay (primers + probe) for P. larvae
Positive Control P. larvae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

preloaderpreloaderpreloaderpreloader