BactoReal® Borrelia burgdorferi sensu lato


BactoReal Borrelia_RTGV



Background: Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause the Lyme disease (Lyme borreliosis). These species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. The Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

BactoReal® Borrelia burgdorferi sensu lato is a detection assay for rapid and sensitive detection of DNA of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex by real-time PCR. Borrelia spielmanii is not well detected with this assay.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Borrelia burgdorferi sensu lato

Product BactoReal® Borrelia burgdorferi sensu lato
Order no. RTGV1270
Unit 100 reactions
Technology Real-time PCR
Target gene ospA gene, detection in FAM channel
Content Assay (pimers + probe) for Borrelia species belonging to the B. burgdorferi sensu lato complex
Positive Control Borrelia
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.