BactoReal® Brachyspira hyodysenteriae

 

BactoReal Brachyspira hyodysenteriae

 

 

Background: Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is an anaerobic intestinal spirochaete and the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira hyodysenteriae is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

BactoReal® Brachyspira hyodysenteriae is a detection assay for DNA of Brachyspira hyodysenteriae by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Brachyspira hyodysenteriae

Product BactoReal® Brachyspira hyodysenteriae
Order no. RTGV610
Unit 100 reactions
Technology Real-time PCR
Target gene nox gene, detection in FAM channel
Content Assay (pimers + probe) for Brachyspira hyodysenteriae
Positive Control Brachyspira hyodysenteriae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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