BactoReal® Brachyspira pilosicoli


BactoReal Brachyspira pilosicoli


Background: Brachyspira pilosicoli (previously called Serpulina pilosicoli) is an anaerobic intestinal spirochaete. This bacterium colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS). Brachyspira pilosicoli is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

BactoReal® Brachyspira pilosicoli is a detection assay for DNA of Brachyspira pilosicoli by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Brachyspira pilosicoli

Product BactoReal® Brachyspira pilosicoli
Order no. RTGV620
Unit 100 reactions
Technology Real-time PCR
Target gene nox gene, detection in FAM channel
Content Assay (pimers + probe) for Brachyspira pilosicoli
Positive Control Brachyspira pilosicoli
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.