BactoReal® Brachyspira hyo/pilo

 

 

Background: Brachyspira are anaerobic intestinal spirochaetes. Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira pilosicoli (previously called Serpulina pilosicoli) colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS). Brachyspira are anaerobic bacteria but are aerotolerant due, at least in part, to high NADH oxidase activity.

BactoReal® Brachyspira hyo/pilo is a detection assay for DNA of B. hyodysenteriae and B. pilosicoli by real-time polymerase chain reaction. Other Brachyspira species are not detected by this test.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Brachyspira hyo/pilo

Product BactoReal® Brachyspira hyo/pilo
Order no. RTGV600
Unit 100 reactions
Technology Real-time PCR
Target gene nox gene, detection in FAM channel
Content Assay (pimers + probe) for B. hyodysenteriae + B. pilosicoli
Positive Control B. hyodysenteriae
Positive Control B. pilosicoli
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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