BactoReal® Clostridium perfringens

 

BactoReal Clostridium perfringens_RTGVBakterien Assay Vet FAM

 

Background: Clostridium perfringens is a spore-forming gram-positive bacterium present in many environmental sources as well as in the intestines of humans and animals. Under certain predisposing conditions, Clostridium perfringens proliferates rapidly, produces toxins and causes disease such as gas gangrene, necrotic enteritis and enterotoxemia. This bacterium is an important cause of food poisoning. Stress and the feed composition are believed to be of major importance for the development of disease. Clostridium perfringens strains are classified into five types (A–E) on the basis of their ability to produce the major lethal toxins alpha (cpa), beta (cpb), epsilon (etx), and iota (iap) which give the cytotoxic and necrotic activity associated with infection. In addition, strains of C. perfringens may also produce a number of other toxins, including enterotoxin (cpe), beta-2 toxin (cpb2) or theta toxin.

BactoReal® Clostridium perfringens is a detection assay for rapid and sensitive detection of DNA of Clostridium perfringens by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Clostridium perfringens

Product BactoReal® Clostridium perfringens
Order no. RTGV928
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rRNA gene, detection in FAM channel
Content Assay (primers + probe) for C. perfringens
Positive Control C. perfringens
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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