BactoReal® Corynebacterium pseudotuberculosis

 

BactoReal Corynebacterium pseudotuberculosis

 

Background: Corynebacterium pseudotuberculosis is a Gram-positive bacterium and the aetiological agent of external or cutaneous abscessation, lymphangitis, and internal abscessation affecting sheep, goats, horses, cattle and rarely humans as well. Corynebacterium pseudotuberculosis produces an exotoxin called phospholipase D that enhances dissemination of the bacteria by damaging endothelial cells and increasing vascular permeability.

BactoReal® Corynebacterium pseudotuberculosis is a detection assay for rapid and sensitive detection of DNA of Corynebacterium pseudotuberculosis by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Corynebacterium pseudotuberculosis

Product BactoReal® Corynebacterium pseudotuberculosis
Order no. RTGV919
Unit 100 reactions
Technology Real-time PCR
Target gene pld gene, detection in FAM channel
Content Assay (pimers + probe) for C. pseudotuberculosis
Positive Control C. pseudotuberculosis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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