BactoReal® Coxiella burnetii

 

BactoReal Coxiella burnetii_RTGV

 

 

Background: Coxiella burnetii (C. burnetii) is a Gram-negative, obligate intracellular, zoonotic bacterial pathogen, which is the causative agent of Q fever (Coxiellosis). The disease is spread through inhalation of a spore-like cell variant or close contact with body fluids or products from contaminated animals. Cattle, sheep and goats represent the main animal reservoir. Seropositivity of ruminants for C. burnetti is associated with abortion, stillbirth and fertility problems. The high resistance to standard disinfectants and environmental influences renders disease control difficult. Antibiotic treatment and vaccination against Q fever is available. 

BactoReal® Coxiella burnetii is a detection assay for DNA of Coxiella burnetii by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Coxiella burnetii

Product BactoReal® Coxiella burnetii
Order no. RTGV231
Unit 100 reactions
Technology Real-time PCR
Target gene IS1111 region, detection in FAM channel
Content Assay (pimers + probe) for Coxiella burnetii
Positive Control Coxiella burnetii
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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