BactoReal® Enterococcus spp.


BactoReal Enterococcus spp_For research only


Background: The genus Enterococcus includes more than 17 species, until 1984 Enterococcus species were classified as Group D Streptococcus. This genus belongs to lactic acid bacteria of the phylum Firmicutes. Enterococci are Gram-positive cocci, they are facultative anaerobic organisms that can survive and grow in many environments. They are part of the normal intestinal flora of humans and animals but have also been found in soil, water, plants and insects. Enterococci are important pathogens responsible for serious infections. In cattle, enterococci have been associated with diarrhea in calves and bovine mastitis in dairy cattle.

BactoReal® Enterococcus spp. is a detection assay for rapid and sensitive detection of DNA of Enterococcus species by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Enterococcus spp.

Product BactoReal® Enterococcus spp.
Order no. RTGV905
Unit 100 reactions
Technology Real-time PCR
Target gene 23S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Enterococcus species
Positive Control Enterococcus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.