BactoReal® Escherichia coli


BactoReal Escherichia coli



Background: Escherichia coli is a gram negative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. The dxs gene (1-deoxyxylulose-5-phosphate synthase gene) can be found in E. coli and Shigella strains.

BactoReal® Escherichia coli is a detection assay for rapid and sensitive detection of DNA of Escherichia coli and Shigella by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Escherichia coli

Product BactoReal® Escherichia coli
Order no. RTGV914
Unit 100 reactions
Technology Real-time PCR
Target gene dxs gene, detection in FAM channel
Content Assay (pimers + probe) for Escherichia coli
Positive Control Escherichia coli
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.