BactoReal® European Foulbrood


BactoReal European FoulbroodBakterien Assay Vet FAM


Background: European foulbrood (EFB) is a bacterial brood disease of honey bees caused by the Gram-positive bacterium Melissococcus plutonius. The disease occurs throughout the world. It may cause serious losses of brood. Larvae get infected by consuming contaminated food fed by the nurse bees. The bacteria multiply within the larval gut, competing with it for its food. Larvae that die from the disease have been starved of food.

BactoReal® European Foulbrood is a detection assay for rapid and sensitive detection of DNA of Melissococcus plutonius by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit European Foulbrood

Product BactoReal® European Foulbrood
Order no. RTGV923
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in FAM channel
Content Assay (primers + probe) for M. plutonius
Positive Control M. plutonius
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.