BactoReal® Haemophilus parasuis

 

BactoReal Haemophilus parasuis

 

Background:Haemophilus parasuis is a Gram-negative bacterium (member of the Pasteurellaceae) of the upper respiratory tract of swine. It is the aetiological agent of Gl├Ąssers disease, a severe systemic disease characterized by fibrinous polyserositis, pericarditis, arthritis and meningitis. In addition to the acute form of the disease, Haemophilus parasuis is an important component of the Porcine Respiratory Disease Complex (PRDC). Fifteen serovars of H. parasuis have been reported, but a high percentage of the evaluated isolates are nontypeable. Isolates from the upper respiratory tract are mainly apathogenic strains of clinically healthy swine.

BactoReal® Haemophilus parasuis is a detection assay for DNA of Haemophilus parasuis by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Haemophilus parasuis

Product BactoReal® Haemophilus parasuis
Order no. RTGV500
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Haemophilus parasuis
Positive Control Haemophilus parasuis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

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