BactoReal® Lawsonia intracellularis

 

BactoReal Lawsonia intracellularis

 

Background: Lawsonia intracellularis is a Gram-negative and obligate intracellular bacterium and the causative agent of porcine proliferative enteropathy (PPE), which is a transmissible enteric disease of growing pigs characterized by adenomatous proliferation of immature intestinal epithelial cells in the distal small intestines, particularly in the ileum. The infection is widely distributed among pig herds in many pig-producing countries, and affected pigs may exhibit impaired growth and diarrhoea.

BactoReal® Lawsonia intracellularis is a detection assay for DNA of Lawsonia intracellularis by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Lawsonia intracellularis

Product BactoReal® Lawsonia intracellularis
Order no. RTGV300
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Lawsonia intracellularis
Positive Control Lawsonia intracellularis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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