BactoReal® Mannheimia haemolytica


BactoReal Mannheimia haemolytica


Background: Mannheimia haemolytica (formerly Pasteurella haemolytica) is an opportunistic pathogen of cattle, sheep and other ruminants. It causes epizootic pneumonia in cattle known as shipping fever, transit fever or pneumonic pasteurellosis. Mannheimia haemolytica is considered as part of the normal bacterial flora found in the upper respiratory tract of most cattle but is not considered as normal flora of the lungs. It gains access to the lungs when host defenses are compromised by stress or infection with respiratory viruses or mycoplasma.

BactoReal® Mannheimia haemolytica is a detection assay for rapid and sensitive detection of DNA of Mannheimia haemolytica by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Mannheimia haemolytica

Product BactoReal® Mannheimia haemolytica
Order no. RTGV222
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Mannheimia haemolytica
Positive Control Mannheimia haemolytica
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an┬áingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.