BactoReal® Mycobacterium avium ssp. paratuberculosis

 

BactoReal Mycobacterium avium

 

Background: Mycobacterium avium ssp. paratuberculosis (MAP) causes Johne’s disease, a chronic gastroenteritis affecting primarily ruminants and many other species. This disease is one of the most widespread and economically important diseases of ruminants. It has a relatively long incubation period which is characterized by the excretion of MAP in faeces for months and years before first clinical symptoms develop.

BactoReal® Mycobacterium avium ssp. paratuberculosis is a detection assay for rapid and sensitive detection of DNA of Mycobacterium avium ssp. paratuberculosis by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Mycobacterium avium ssp. paratuberculosis

Product BactoReal® Mycobacterium avium ssp. paratuberculosis
Order no. RTGV240
Unit 100 reactions
Technology Real-time PCR
Target gene insertion sequence IS900, detection in FAM channel
Content Assay (pimers + probe) for Mycobacterium avium ssp. paratuberculosis
Positive Control Mycobacterium avium ssp. paratuberculosis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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