BactoReal® Mycobacterium tuberculosis complex


BactoReal Mycobacteria tuberculosis complex



Background: Tuberculosis in humans is caused by members of the Mycobacterium tuberculosis complex (MTBC). The eight closely related species of the MTBC have a wide range of natural hosts, including human hosts (M. tuberculosis, M. africanum, M. canettii), bovine hosts (M. bovis), caprine hosts (M. caprae), rodent hosts (M. microti), and pinniped hosts (M. pinnipedii). Bovine tuberculosis remains an important disease in many countries of the world, causing significant economic losses.

BactoReal® Mycobacterium tuberculosis complex is a detection assay for rapid and sensitive detection of DNA of Mycobacterium species belonging to the Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis,  M. caprae, M. canetti, M. africanum and M. microti) by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Mycobacterium tuberculosis complex

Product BactoReal® Mycobacterium tuberculosis complex
Order no. RTGV250
Unit 100 reactions
Technology Real-time PCR
Target gene ITS2 region, detection in FAM channel
Content Assay for Mycobacterium tuberculosis complex
Positive Control Mycobacterium tuberculosis
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an┬áingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.