BactoReal® Mycoplasma hyopneumoniae


BactoReal Mycoplasma hyopneumoniae



Background: Mycoplasma hyopneumoniae is a primary pathogen of respiratory disease in pigs. This bacterium is one of the most important causative agents of the enzootic pneumonia (EP) and of the porcine respiratory disease complex (PRDC).

BactoReal® Mycoplasma hyopneumoniae is a detection assay for DNA of Mycoplasma hyopneumoniae by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Mycoplasma hyopneumoniae

Product BactoReal® Mycoplasma hyopneumoniae
Order no. RTGV210
Unit 100 reactions
Technology Real-time PCR
Target gene rpoB gene, detection in FAM channel
Content Assay (pimers + probe) for Mycoplasma hyopneumoniae
Positive Control Mycoplasma hyopneumoniae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

Ingenetix ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.