BactoReal® Mycoplasma synoviae

 

BactoReal Mycoplasma synoviae

 

 

Background: Avian mycoplasmosis is caused by several pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae are the most important avian pathogens. In addition, Mycoplasma meleagridis and Mycoplasma iowae can also cause disease in poultry, but Mycoplasma gallisepticum and Mycoplasma synoviae are considered to be the main species of pathogenic mycoplasmas. Mycoplasma gallisepticum and Mycoplasma synoviae cause chronic respiratory disease (CRD) and infectious synovitis of chickens and turkeys especially in the presence of stresses or other respiratory pathogens, causing economic losses due to decrease in growth rate, loss of weight and reduced egg production.

BactoReal® Mycoplasma synoviae is a detection assay for rapid and sensitive detection of DNA of Mycoplasma synoviae by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Mycoplasma synoviae

Product BactoReal® Mycoplasma synoviae
Order no. RTGV907
Unit 100 reactions
Technology Real-time PCR
Target gene vlhA gene, detection in FAM channel
Content Assay (pimers + probe) for Mycoplasma synoviae
Positive Control Mycoplasma synoviae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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