BactoReal® Pasteurella multocida

BactoReal Pasteurella multocida



Background: The Gram-negative bacterium Pasteurella multocida is able to cause disease in a wide range of animal species and in humans. Pasteurella multocida can cause fowl cholera disease in poultry, hemorrhagic septicemia and shipping fever in cattle, atrophic rhinitis in pigs and is the most common cause of respiratory disease, primarily rhinitis, in rabbits. Although Pasteurella multocida is part of the flora in the respiratory tract of pigs, the bacterium can induce hemorrhagic septicemia or, usually as a secondary pathogen invading lungs injured by other bacteria or viruses, pneumonia. A subset of Pasteurella multocida isolates are critical agents in an upper respiratory disease, progressive atrophic rhinitis (PAR) in pigs.

BactoReal® Pasteurella multocida is a detection assay for rapid and sensitive detection of DNA of Pasteurella multocida (subsp. multocida, gallicida, septica and tigris) by real-time PCR. For detection of toxigenic P. multocida strains associated with PAR ingenetix offers BactoReal® Pasteurella multocida toxA. 

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Pasteurella multocida

Product BactoReal® Pasteurella multocida
Order no. RTGV444
Unit 100 reactions
Technology Real-time PCR
Target gene 16S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Pasteurella multocida
Positive Control Pasteurella multocida
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an┬áingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.