BactoReal® Pasteurella multocida toxA

BactoReal Pasteurella multocida_toxA

 

 

Background: Pasteurella multocida is a Gram-negative bacterium and part of the flora in the respiratory tract of pigs. The bacterium induces hemorrhagic septicemia or, usually as a secondary pathogen invading lungs injured by other bacteria or viruses, pneumonia. A subset of P. multocida isolates are critical agents in an upper respiratory disease, progressive atrophic rhinitis (PAR). Pasteurella multocida strains associated with PAR usually produce a dermonecrotic toxin, which is encoded by the toxA gene. The toxA protein of these isolates acts as an essential virulence factor inducing turbinate atrophy and poor weight gains in pigs. Non-toxigenic P. multocida strains are not usually associated with PAR. Therefore, confirmation of toxin production is important for the diagnosis and control of the disease.

BactoReal®  Pasteurella multocida toxA is a detection assay for DNA of toxigenic Pasteurella multocida strains by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Pasteurella multocida toxA

Product BactoReal® Pasteurella multocida toxA
Order no. RTGV400
Unit 100 reactions
Technology Real-time PCR
Target gene toxA gene, detection in FAM channel
Content Assay (pimers + probe) for Pasteurella multocida toxA
Positive Control Pasteurella multocida toxA
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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