BactoReal® Salmonella enterica

 

BactoReal Salmonella entericaBakterien Assay Vet FAM

 

 

Background: Salmonella is a genus of Gram-negative bacteria of the Enterobacteriaceae family. This genus consists of only two species, Salmonella enterica and Salmonella bongori. Salmonella bongori mostly infects cold blooded animals. Salmonella enterica has six subspecies and more than 2500 serovars. Subspecies are S. enterica subsp. arizonae, S. enterica subsp. diarizonae, S. enterica subsp. enterica, S. enterica subsp. houtenae, S. enterica subsp. indica and S. enterica subsp. salamae. Salmonella infections in warm-blooded animals are generally caused by strains from S. enterica subspecies enterica. Typhoidal serovars include Salmonella Typhi and Salmonella Paratyphi, which only infect humans. Nontyphoidal serovars are more common, and usually cause self-limiting gastrointestinal disease. They can infect a range of animals, and are zoonotic. Although many other Salmonella serovars may cause enteric disease, the more common ones in cattle are Salmonella Typhimurium, Salmonella Dublin and Salmonella Newport. In sheep and goat the most common are Salmonella Typhimurium, Salmonella Dublin, Salmonella Abortusovis, Salmonella Anatum and Salmonella Montevideo. In pigs the most common are Salmonella Typhimurium and Salmonella Choleraesuis, and in poultry Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum and Salmonella Pullorum.

BactoReal® Salmonella enterica is a detection assay for rapid and sensitive detection of DNA of Salmonella enterica by real-time PCR.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Salmonella enterica

Product BactoReal® Salmonella enterica
Order no. RTGV926
Unit 100 reactions
Technology Real-time PCR
Target gene inv A gene, detection in FAM channel
Content Assay (primers + probe) for S. enterica
Positive Control S. enterica
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

 ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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