BactoReal® Staphylococcus aureus


BactoReal Staphylococcus aureus


Background: Staphylococcus aureus is a Gram-positive bacterium. In healthy animals, S. aureus colonization of the skin, intestinal tract, or nasopharynx is generally asymptomatic. However, depending on the strain, S. aureus is capable of secreting several exotoxins. Many of these toxins are associated with specific diseases. By producing a toxin, S. aureus is also a common cause of foodborne illness. In cattle, S. aureus causes one of the most common types of chronic mastitis. In humans, S. aureus is a frequent commensal of the skin, but can cause a range of illnesses from minor skin infections, to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome and sepsis.

BactoReal® Staphylococcus aureus is a detection assay for DNA of Staphylococcus aureus by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Staphylococcus aureus

Product BactoReal® Staphylococcus aureus
Order no. RTGV800
Unit 100 reactions
Technology Real-time PCR
Target gene nuc gene, detection in FAM channel
Content Assay (pimers + probe) for Staphylococcus aureus
Positive Control Staphylococcus aureus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.