BactoReal® Staphylococcus spp.


BactoReal Staphylococcus spp_For research only


Background: The genus Staphylococcus includes more than 50 different species. Staphylococci are commensals on skin and upper respiratory tracts of mammals and birds. However, they can also cause various infections of the skin and a wide variety of diseases in humans and animals through either toxin production or penetration. Foreign bodies, such as sutures, indwelling catheters, and implanted joints, are extremely susceptible to Staphylococcus epidermidis colonization, and often serve as the point of entry for infection. Another common path of infection is the respiratory tract, where the bacterium causes pneumonia. Other diseases caused by staphylococci include boils, sinusitis, emesis, diarrhea, endocarditis, scalded skin syndrome, osteomyelitis, urinary tract infection, toxic shock syndrome and food poisoning. Staphylococci, mainly Staphylococcus aureus can also cause bovine mastitis.

BactoReal® Staphylococcus spp. is a detection assay for rapid and sensitive detection of DNA of Staphylococcus species by real-time PCR. This assay has an analytical sensitivity of 50 copies/reaction, as in negative samples the presence of contaminating staphylococcal DNA in the extraction or amplification reagents results in weak positive amplification. High concentrations of some non-Staphylococcus species might lead to weak cross reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Staphylococcus spp.

Product BactoReal® Staphylococcus spp.
Order no. RTGV804
Unit 100 reactions
Technology Real-time PCR
Target gene 23S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Staphylococcus spp.
Positive Control Staphylococcus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.