BactoReal® Streptococcus agalactiae


BactoReal Streptococcus agalactiae_RTGV



Background: Streptococcus agalactiae (also known as Group B streptococcus or GBS) is a beta-hemolytic Gram-positive streptococcus. It is pathogenic primarily for animals, but can also infect humans. Since S. agalactiae strains isolated from animals can be differentiated from those isolated from humans, transmission from animals to humans is rare. In cattle it causes mastitis, an infection of the udder which leads to diminished milk production. Transmission occurs during milking. Streptococcus agalactiae secretes a protein (CAMP factor) which causes haemolysis in the presence of beta-toxin of Staphylococcus aureus in blood agar.

BactoReal® Streptococcus agalactiae is a detection assay for DNA of Streptococcus agalactiae by real-time polymerase chain reaction.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Streptococcus agalactiae

Product BactoReal® Streptococcus agalactiae
Order no. RTGV900
Unit 100 reactions
Technology Real-time PCR
Target gene cfb gene, detection in FAM channel
Content Assay (pimers + probe) for Streptococcus agalactiae
Positive Control Streptococcus agalactiae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 1.2/1.5/2.0/480 and MX3005P. Assay can also be used with other real-time PCR instruments.


Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).

ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.