BactoReal® Streptococcus dysgalactiae

 

BactoReal Streptococcus dysgalactiae

 

 

Background: Streptococcus dysgalactiae is a Gram-positive bacterium belonging to the group C streptococci. It is known to cause clinically acute and subclinical mastitis in cows, ewes and goats, as well as polyarthritis.

BactoReal® Streptococcus dysgalactiae is a detection assay for DNA of Streptococcus dysgalactiae by real-time polymerase chain reaction. This test is performed in a multiplex real-time PCR format to enable a differentiation between S. dysgalactiae from other Streptococcus species, especially S. canis.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Streptococcus dysgalactiae

Product BactoReal® Streptococcus dysgalactiae
Order no. RTGV903
Unit 100 reactions
Technology Multiplex real-time PCR
Target gene 23S rDNA gene, detection in FAM channel
16S rDNA gene, detection in VIC/HEX channel
Content 23S assay (pimers + probe) for S. dysgalactiae + S. canis
16S assay (pimers + probe) for Streptococcus non S. canis
Positive Control Streptococcus dysgalactiae
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) ÔÇô not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real 3 (order no. RTGMCR-3) contains primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assay 3 (order no. RTGMIPC-3) contains primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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