BactoReal® Streptococcus spp.

 

BactoReal Streptococcus spp 

 

 Background: Streptococcus is a genus of Gram-positive bacteria belonging to the Firmicutes and the lactic acid bacteria group. There are currently over 50 species known in this genus. Many streptococcal species are nonpathogenic and form part of the commensal animal or human microbiome of the mouth, skin, intestine, and upper respiratory tract. Certain Streptococcus species are responsible for streptococcal pharyngitis, pink eye, meningitis, bacterial pneumonia, endocarditis, erysipelas, necrotizing fasciitis and bovine mastitis.

BactoReal® Streptococcus spp. is a detection assay for rapid and sensitive detection of DNA of Streptococcus species by real-time PCR. This assay has an analytical sensitivity of 50-100 copies/reaction, as in negative samples the presence of contaminating DNA of streptococci in the extraction or amplification reagents results in weak positive amplification.

Specificity and sensitivity: See product description

Also available as kitversion: BactoReal® Kit Streptococcus spp.

Product BactoReal® Streptococcus spp.
Order no. RTGV904
Unit 100 reactions
Technology Real-time PCR
Target gene 23S rDNA gene, detection in FAM channel
Content Assay (pimers + probe) for Streptococcus species
Positive Control Streptococcus
Amplification mix & IPC Not included, see product description
PCR-Platforms Evaluated for ABI PRISM® 7500, LightCycler® 480 and MX3005P. Assay can also be used with other real-time PCR instruments.

 

Internal Positive Control (IPC) – not included, optional: For exclusion of false-negative interpretation of results caused by PCR inhibition an ingenetix internal positive control assay (ControlReal tests, or Internal Positive Control Assays) can be optionally performed in a multiplex PCR, depending on the PCR-platform. See product description.
– Control
Real tests (order no. RTGMCR-1, 2 or 3) contain primers, a probe and an internal control PCR target included in the assay mix.
Internal Positive Control Assays (order no. RTGMIPC-1, 2 or 3) contain primers, a probe and an internal control PCR target in an extra tube (for control of DNA extraction and PCR inhibition).


ViroReal®, BactoReal® and ParoReal Assays detecting viral, bacterial and parasitic DNA are optimized to run under the same thermal cycling conditions and with the same amplification mix.

 

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